How to Use a Micropipette?


Micropipette is a laboratory equipment piece designed to transfer extremely small amounts of liquid. This equipment is as common as the microscope and has it’s applications in analytical chemistry and micro biology besides being used in medical laboratories. This is used to transfer anywhere between 1 to 1000 micro litres of liquid. That is a small amount of liquid and thus requires a pipette with a great deal of accuracy and precision. In addition it also requires a skilled technician to operate the pipette as the slightest error can cause the reading to differer drastically (specially true for micro pipette since dealing with small amount of liquid). The micro pipette consists of a volume adjustment dial and even a digital volume indicator of an electric pipette.

There are generally two buttons found on the micropipette, the plunger button and the ejector button. The ejector button is used to release the tip of the pipette that is generally disposable.The tips are generally produced from high quality diamond finish mould using special medical grade polypropylene with high transparency.

Micro pipettes come in several different ranges of volumes and it is important not to exceed the upper or lower limits of the pipette. The micro pipette has an adjustment dial that can be used to set the volume of liquid entering the tip. The digital dial can be utilized to read the settings. It is important to keep the tip sterile and not let it come in contact with anything else during the process. The plunger will stop at two different positions when it is depressed. You need to depress the plunger till the initial resistance is felt and then insert the tip into the solution. Again care should be taken not to submerge the tip entirely but to place it just below the surface. The plunger needs to be carefully released now allowing the pipette to fill up. Mind you if the liquid is vicious it will take longer to fill up. Air bubbles should be avoided as they will only disrupt the reading. It is important never to point the tip up since this can cause the liquid to run down into the pipette destroying it. You may now depress the plunger to the initial resistance point to discharge the solution and after waiting for a second or depress the plunger completely to discharge all the measured volume of liquid. The tip then needs to be discarded by using the eject button provided on the micropipette.

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